FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22150692
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG141200.061.5460494
CAAGTCG110050.031.81735494
TAAGTCG40450.026.6081694
GAAGTCG149850.025.81314894
AAAGTCG56600.018.35159194
GCGAAAT30700.017.6058651
GTTTTTT265000.015.6252831
GCCGAAT51300.013.9259261
CGAATCG18700.013.5721943
TTTTTTC358000.013.5354752
GCGGAAT41100.013.3795621
GAAAAAT206450.012.6577871
AAAAATC201350.012.4882052
GTTTTAT147400.012.4036651
AAATCCA193350.012.2270514
GCGGTAT31250.011.88161
CGCGAAC14250.011.8736851
GTTTTTC163150.011.782411
CGAAAAC37200.011.7500011
GTTTTAC65250.011.7409961
Produced by FastQC (version 0.11.5)