FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22150692
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG169000.051.00473894
CAAGTCG135900.029.5003794
TAAGTCG41800.025.52392494
GAAGTCG142850.023.06405694
AAAGTCG65550.015.55911694
CGAAAAC38300.014.6031351
GTTTTTT255350.014.3383611
CGCAAAT33550.013.44858551
AAATCGA33000.013.387884
GCCGAAT50850.013.2173061
CGCGAAC15700.013.1719761
GTTTAAT77350.013.1247591
GCGGAAT39300.012.9160311
AAATCCA195750.012.6053654
GCGGTAT30550.012.1538471
CGGAAAT42500.012.0541181
AAAAATC195150.011.7289272
GCGAAAT26950.011.3358081
GTTTTAC69900.011.2288991
CGCAATT20950.011.2171841
Produced by FastQC (version 0.11.5)