FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22606873
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114850.059.21550494
CAAGTCG89000.029.15056494
TAAGTCG33600.026.437594
GAAGTCG135450.023.24843294
AAAGTCG46300.017.35853294
CGCGTAA8000.014.6875011
GTTCGAT30900.013.0809061
AATCGTA22900.012.9301325
GTCGAAT14750.011.4711881
CGCGAAT16100.011.3850941
ATCGTAT18350.011.2697556
TTAGCGA23450.011.2238828
TAGCGAG26850.011.202989
TTCGATT34650.011.1226562
TCGGATT25600.010.8320312
GTCGGAT27350.010.4826341
GTCCGAT14800.010.4797311
GTTTATT107850.010.4589721
GTCGGTT51700.010.3636361
CGCGTAT4651.4776035E-410.1075281
Produced by FastQC (version 0.11.5)