FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22102685
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG79900.046.64706494
CAAGTCG64300.024.55987794
TAAGTCG29000.019.12413894
GAAGTCG101250.016.33975494
GCGAAAT27050.015.637711
AAAGTCG42450.015.38987194
GTTTTTT296650.015.1940011
TTTTTTC401200.014.690432
CGAAAAT38950.013.6354311
GTCGAAT14350.013.4285721
AAATCGA32800.013.1829274
AAAAATC229100.013.0886082
GAAAAAT237450.012.8460731
GTTTTTC176650.012.7710161
GTTTAAT94750.012.5002651
CTCGATT42050.012.406661
GTTTTAC68850.011.946261
AAATCCA208250.011.8713084
CGGAAAT44950.011.8153511
CTCGAAT35450.011.7997181
Produced by FastQC (version 0.11.5)