FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22102685
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG103650.035.59575794
CAAGTCG84200.021.32304294
TAAGTCG27850.019.74506494
GAAGTCG103350.015.73488294
CGAAAAC31800.014.6320751
GTTTTTT317800.014.1384531
CTCGAAT37200.012.8870971
AAATCGA33200.012.7409654
CTTAGAT56300.012.4387211
CGGAAAT39900.012.3684211
GTTTAAT91450.012.334611
CGCAATT20950.012.1145591
CGAAAAT40300.012.0124071
GTTTTAT161550.011.9281951
AAATCCA196400.011.8935854
GTTTTTC184200.011.7372431
CGCAAAC36750.011.5102041
CTCAGAT179350.011.451911
GCGAAAT25900.011.4324331
AAAAATC209850.011.4224452
Produced by FastQC (version 0.11.5)