FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21530495
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125150.056.44507294
CAAGTCG96150.030.4045894
TAAGTCG40050.023.0012594
GAAGTCG138500.022.73646494
AAAGTCG55900.015.30232694
GTTTTTT263550.014.5520771
TTTTTTC369250.013.9122552
TATACGC10150.013.8916277
TACGCTA12000.013.3166679
CGCAAAT30200.012.9172181
TCGGATC44100.012.682542
GTTTTAC77100.012.3748381
CGGATAT25450.012.3732811
GTTTAAT85150.012.3640641
CGAAAAT36100.012.1080341
AAAAATC204050.012.0004912
GAAAAAT214400.011.9034521
CGCGGAT24100.011.8962661
AAATCCA189700.011.6942554
GCCGAAT46350.011.5598711
Produced by FastQC (version 0.11.5)