FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21530495
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG144400.049.73407794
CAAGTCG107650.029.16488694
GAAGTCG134000.022.48283694
TAAGTCG37500.022.30933494
AAAGTCG58700.014.81260894
GTTTTTT265500.014.1973651
CGAAAAC36400.013.4285731
CGGATAT26750.011.9476641
CGCAAAT32850.011.8751911
CGCAAAC42300.011.6666681
CTTTAAT111650.011.2816851
CTCGAAT40150.011.2378591
GGCGAAT30100.010.9302321
AATCGTA18200.010.8461545
AAATCCA182500.010.8164394
CGCAATT21850.010.755151
CTCAGAT156100.010.7488791
GTTTTAT148700.010.7148621
CGAATCG16900.010.5680483
GTTTAAT83150.010.5135311
Produced by FastQC (version 0.11.5)