FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22141530
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124150.057.12686594
CAAGTCG97500.029.88717794
TAAGTCG35650.027.42215794
GAAGTCG138600.024.1103994
AAAGTCG50750.016.20689694
GTTTTTT292000.015.0979451
CGAAAAT34600.013.4479771
TTTTTTC368800.013.3175172
CGAATCG16400.012.3231723
TTTTTTG427400.012.1073942
AATCGTA19900.012.0452265
CGGAAAT47500.011.9726311
GTTTAAT91050.011.6661171
TCGGATC39350.011.5857692
AAATCCA196650.011.4960584
AAAAATC202650.011.4803862
CGAAGAT81200.011.2869451
TCGAATC17700.011.1525432
CTCGATT34600.011.1387281
GAAAAAT212000.010.9518861
Produced by FastQC (version 0.11.5)