FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22141530
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG149700.046.968694
CAAGTCG115000.027.9547894
TAAGTCG39550.025.431194
GAAGTCG134450.021.77835794
AAAGTCG59050.015.04318494
GTTTTTT282050.013.8308821
CGAAAAC30400.012.5230261
CGAAAAT38900.011.9614391
CGCGTAT4751.1837856E-611.8736841
GGAAAAT163400.011.5055081
AAATCGA30900.011.4077674
GTCGGAT38100.011.22572194
CGAATAT13400.011.2238811
CGGAAAT45300.010.9977921
GGCGAAT52650.010.8015191
CGCGGAT23800.010.6638651
GTCGATT19500.010.6051281
GTTTTCT214600.010.6001861
CGAAGAT79350.010.48393251
AAATCCA201100.010.4704124
Produced by FastQC (version 0.11.5)