FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21756187
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104150.051.53528694
CAAGTCG81450.026.5438994
TAAGTCG32150.025.87558294
GAAGTCG118100.020.61473394
AAAGTCG45300.016.70419394
GTTTTTT305850.015.2133391
GTTTTAC71950.014.763031
TTTTTTC414800.014.7186582
CGAAAAT34600.014.3988441
GCGAAAT30800.013.4285711
CGGATAT23200.013.370691
TTTTTTG462200.013.3312432
GAAAAAT224450.013.2760081
TTTTTTA414400.012.8955112
GGAAAAT173900.012.7837821
CGCATAT8600.012.5697671
CGAATCG16200.012.4753083
CGCAATT22650.012.450331
AAATCCA196300.012.4263874
GTTTTTC168050.012.4177321
Produced by FastQC (version 0.11.5)