FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22648208
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG113150.056.94829694
CAAGTCG88900.029.44769394
TAAGTCG33200.026.1897694
GAAGTCG133350.019.66704294
GTTCGAT30600.015.9738561
AAAGTCG46050.015.30944694
TTCGATT32100.013.763242
AATCGTA22350.012.4071595
GTCGTTT20400.011.5196081
CGCTAAT8257.2759576E-1211.393941
CGCGTAT6252.147317E-811.281
TTAGCGA23400.010.8461538
TAGCGAG26650.010.7579749
GGCGAAT50850.010.6293021
TATACGG19050.010.60892334-35
GTCGAAA35950.010.5897081
CGTATCG16100.010.5093173
GTCGGAA38600.010.2279791
GTCGGTT52400.010.2251911
GGCGATT38750.010.1883881
Produced by FastQC (version 0.11.5)