FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22951069
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG109800.053.76320694
CAAGTCG90100.028.3773694
TAAGTCG33800.023.22189394
GAAGTCG118600.019.22006894
AAAGTCG51000.015.0215794
CGCGTAT4608.033694E-712.260871
CGTATCG20750.010.8722913
GGCGAAT51500.010.7689321
AATCGTA23850.010.6415095
GTTCGAT27650.010.5388791
GTATCGA31550.010.2789234
TTAGCGA20850.010.1438868
CTCGGTT37600.010.1250011
GTCGGTT46600.09.6824041
TAGCGAG27300.09.64102659
GTTTATT109250.09.5935931
TTCGATT30600.09.5228772
GTCGATT21850.09.2494291
GTCCGAT15800.09.2215191
CGCGTAA7201.7967468E-69.1388891
Produced by FastQC (version 0.11.5)