FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22951069
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126850.046.68506294
CAAGTCG102450.027.84675694
TAAGTCG32200.023.50000294
GAAGTCG117050.018.67150994
AAAGTCG53650.012.877912594
GTTCGAT25650.012.8265111
GTCCGAT18000.012.5333341
CGCGTAT5051.8885657E-712.09900951
CGCGGAT27600.011.920291
TTAGCGA21400.011.2009358
AATCGTA22550.010.2128615
CCGTATC32500.09.9784612
TTCGATT30900.09.8867322
GCGGTAT21500.09.8372091
CGAAGAT76250.09.7390171
CGTATCG20800.09.7163473
GGCGTAT30650.09.5073411
CCCGTAT31650.09.2069511
GCCGGTT47700.09.1635221
TAGCGAG27200.09.1580899
Produced by FastQC (version 0.11.5)