FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22532712
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG150100.047.21918594
CAAGTCG111650.027.90953894
TAAGTCG38250.022.11764794
GAAGTCG130700.019.34659494
AAAGTCG66850.015.32685194
GTTTTTT317000.014.693061
CGAAAAC41850.013.9259261
GCGAAAT31750.013.766931
CGGATAT27700.012.3862811
CTCGTTT38750.011.76516151
GGAAAAT217350.011.7418921
AAATCGA36950.011.70234
GCGAATT24750.011.5838381
GTTTTAT185650.011.569621
AAATCCA207100.011.4379524
CGGAAAT46250.011.3816211
GCCGAAT46350.011.3570661
GCGGTAT30050.011.1048251
GTTTAAT108050.011.0920871
CGCGTAT4701.3623432E-511.01
Produced by FastQC (version 0.11.5)