FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22355507
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128500.058.0824994
CAAGTCG101150.031.45724194
TAAGTCG37400.024.00267494
GAAGTCG140250.022.98894994
AAAGTCG55300.015.80831994
GCGAAAT31300.015.4664541
GTTTTTT303350.015.3232251
TTTTTTC410300.014.3417012
GCCGAAT48600.013.5390951
TCGGATC42600.013.4600942
GCGGTAT30950.013.2116321
AAATCCA199650.012.9476584
TATACGC10350.012.7149757
TACGCTA11200.012.5892869
GAAAAAT237600.012.5016831
CGAAAAC38550.012.4357981
AATCCAC116350.012.2397945
TTTTTTG442400.012.2386992
GTTTTAT167300.012.1643751
AAAAATC230700.012.1421762
Produced by FastQC (version 0.11.5)