FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22355507
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG158000.047.26772394
CAAGTCG117550.027.58825994
TAAGTCG40350.022.83023694
GAAGTCG135450.021.89516494
AAAGTCG65250.015.55862194
GCGGTAT27200.013.6507361
GCGAAAT26950.013.6029691
GTTTTTT300350.013.5671721
GCCGAAT47350.012.4076031
CGAAAAC40950.012.2808291
GTTTAAT90150.012.0953971
CTCGATT38800.011.8711341
CGAATCG16100.011.6770193
AAATCGA35050.011.6661914
CGCGAAC14550.011.6288661
AATCGTA19400.011.6288655
CGGAAAT41650.011.623051
CGAAAAT45400.011.5947131
AAATCCA201500.011.382634
CTCAGAT179800.011.0311461
Produced by FastQC (version 0.11.5)