FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23125215
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG88200.052.75510494
CAAGTCG71200.028.97893194
TAAGTCG29350.021.77853494
GAAGTCG110650.017.3303294
GTCCGAT18000.016.451
AAAGTCG43300.013.45958494
GTTCGAT24100.011.5062241
AATCGTA27700.011.3682315
CGCGATT8751.8189894E-1211.2800011
ATCGTAT21950.010.4920276
GGGAAAT170300.010.3769821
GCGGTAT18200.010.329671
GTTTATT127050.010.284141
CGCGTAT4206.5247697E-410.0714281
CGGAAAT41300.010.0145271
GTCGATT20900.09.8947371
CGCTTAA33550.09.8062644-45
CTCGGTT38550.09.6316471
TTAGCGA16200.09.5740748
GTCGAAT13300.09.5413531
Produced by FastQC (version 0.11.5)