FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22778944
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108900.035.13131394
TAAGTCG28100.022.91459394
CAAGTCG81400.020.38206394
CGACCTA13150.016.083654
GAAGTCG104950.015.13673294
GTTCGAT30800.013.4285721
AATCGTA18650.012.3485265
CGCGGTT37100.011.9083571
TTCGATT31400.011.67515852
CGTATCG21200.011.3066043
GTCGTTT20850.011.2709841
TATACGG17450.010.638967534-35
GGCGATT43500.010.5885051
GTCCGAT16350.010.3486241
AAAGTCG46000.010.31956594
CGCTTAA32800.010.10213544-45
TACGCAC23300.010.0858379
GTTTATT117900.09.846481
CGCGGAT25100.09.7370521
CGCGCAT14200.09.5985911
Produced by FastQC (version 0.11.5)