FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22839483
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124850.060.7216794
CAAGTCG106300.031.08278594
TAAGTCG35100.027.71794994
GAAGTCG129650.021.13459494
AAAGTCG50000.015.0494
TTAGCGA18950.014.8812688
TAGCGAG24550.012.4439939
GTCGAAT13500.012.1851861
GCCGGTT57950.012.0034521
AATCGTA21050.011.6104515
GCCGAAT43750.011.0651441
CTCGGTT36950.010.9391071
GTCGTTT22000.010.8954541
GGCGATT39500.010.4708861
GCGGGTT41850.010.4444451
GGCGAAT38700.010.4444451
GTCGGAA35100.010.4444451
GCGGTAT23700.010.1139251
TCGGATT26100.010.0842912
GGCGGAT91800.09.9324631
Produced by FastQC (version 0.11.5)