FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22839483
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146050.051.90756694
CAAGTCG120850.028.62391594
TAAGTCG33650.024.16344894
GAAGTCG131500.019.22889994
AAAGTCG52950.016.06610194
TTAGCGA20050.011.9551128
GTTCGAT27250.011.55596351
TAGCGAG25400.011.4724419
GCGGTAT22700.010.9735691
TCGGATT25050.010.8822362
GGCGAAT35250.010.8000011
GTCGGAT31350.010.6443391
CGCGTAT5608.5148695E-610.0714281
GTCCGAT15900.010.0503151
CGCATTT34250.010.0175191
GCCGGTT58000.09.8051731
GTCGGTT43650.09.7983971
CGCAATT23100.09.7662341
GTCGTTT22700.09.7312781
GGGAAAT151200.09.7294981
Produced by FastQC (version 0.11.5)