FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21414614
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG121950.060.8167394
CAAGTCG108150.032.1590494
TAAGTCG31450.026.0031894
GAAGTCG129250.022.69090894
AAAGTCG46850.016.25186794
AATCGTA22550.013.9645245
ATCGTAT18050.012.7590036
CGCGTAT4305.065922E-612.0232551
GTCGGTT43900.011.4555811
TCGTATC19750.011.1848117
GTCGCAT22500.011.0711111
GTTCGAT25950.011.0481691
CGCGTGT25000.010.9041
ATAATCG24100.010.531123
CGCAAAT28800.010.4444451
TAATCGT24350.010.4229984
GTCGGAT28500.010.3894741
GGCGAAT44250.010.19660951
GTCGAAA32600.010.0920251
GTCGGGT39650.010.0756621
Produced by FastQC (version 0.11.5)