FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22800783
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107650.053.0469194
CAAGTCG94600.030.00845594
TAAGTCG29750.021.64369894
GAAGTCG119800.019.8906594
AAAGTCG44500.015.20898894
ATCGTAT14850.012.3434336
AATCGTA21950.012.2050115
GTCGGTT46550.011.7121371
GTCCGAT14200.011.5845071
GTTCGAT30500.011.5573761
GTCGGAT29100.011.3058411
GGCGAAT59550.011.2863141
CGTATCG14300.011.1748253
CGCGTAA6503.826426E-810.8461531
TAACGCA21050.010.717339552-53
GGGAAAT163850.010.4412571
TCGGATT25750.010.2213592
ACGTTCG19450.010.14916
GTCGTTT20100.010.0547271
GTCGGAA36000.010.0527771
Produced by FastQC (version 0.11.5)