FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21962439
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG81750.051.28318494
CAAGTCG65450.025.99541794
TAAGTCG28200.021.16666694
GAAGTCG93850.017.52797194
TTTTTTC391050.017.307252
GTTTTTT289550.016.508031
TATACGC7150.015.7762247
AAAGTCG43650.015.6128394
TTTTTTG441350.015.5477522
CTCGATT52800.014.6875011
CTTAGAT60350.014.4855011
TTTTTTA405300.014.2635082
GCGAATT16000.014.0999991
GCGAAAT31200.013.8589741
GAAAAAT226650.013.68631
TACGCTA8000.013.51259
GTTTTAC68600.013.4285711
CGAAAAT39400.013.3604071
AAAAATC219300.013.0948482
AAATCCA194650.012.966354
Produced by FastQC (version 0.11.5)