FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21962439
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115800.036.00086294
TAAGTCG28900.020.49134894
CAAGTCG87600.020.01255694
GAAGTCG103500.017.16521894
CGAATCG15450.015.51456453
GTTTTTT317500.014.9807871
AAAAATC200650.014.3588352
CTCGATT47200.013.641951
GAAAAAT210900.013.6386911
GTTTAAT89050.013.4587321
AAATCCA200800.013.3884464
AAATCGA29050.013.2667824
CGAAAAC31350.012.8931421
AAAATCC192200.012.8870973
GGAAAAT166800.012.59532451
AATCCAC116800.012.5950355
CGAAAAT42200.012.5853091
CGGATAT23200.012.5603451
GTTTTAT163650.012.4356861
CGGAAAT41350.012.2756951
Produced by FastQC (version 0.11.5)