FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21561453
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG177000.059.29435394
TAAGTCG44950.031.57730994
CAAGTCG129050.029.24525694
GAAGTCG164250.027.6992494
AAAGTCG54000.021.06296594
TATACGG17500.013.9657158
TATATCG8450.013.9053267
GCGTATA10050.013.5621896
TAGCGTA8950.013.1284924
GTATACG18550.012.9218337
CGTATAT14750.011.7898317
ATAAGTC52550.011.44814594
CGAAGTC81300.010.81057994
AATAGCG12150.010.4444462
ACAAGTC124450.010.31016492-93
GCAAGTC123250.010.02920994
CGTATCG16950.09.70501536-37
AGAAGTC244800.09.54207694
CGATAAT30050.09.384364
ATAGCGT13550.09.36531453
Produced by FastQC (version 0.11.5)