FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21561453
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT274250.015.2696451
GCGAAAT26250.015.21904851
CGCGTAT3851.4681009E-613.4285721
CGAAAAC30100.012.6478411
AAATCGA31800.012.4150944
CGCGATT5756.2482286E-912.260871
CGCAAAC40050.012.0873911
CGGATAT20350.012.0098281
CGCAATT20750.012.0048191
CGCAAAT28200.012.0000011
CGAAGAT77600.011.9922691
CTCGATT34900.011.9856741
AAATCCA185200.011.8007564
CGCGAAC12350.011.7975721
AAAAATC194150.011.6682992
CGAAAAT39950.011.64705851
GTTTAAT85550.011.646991
CTCGGAT58700.011.5298141
CTCGTTT33100.011.5015111
TATACGC7854.0017767E-1111.3757977
Produced by FastQC (version 0.11.5)