FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22568227
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127550.058.03606494
CAAGTCG99400.031.25452894
TAAGTCG39000.029.5256494
GAAGTCG146800.021.16280794
AAAGTCG54200.017.34317494
GTCGGTT43500.014.0459781
GTTTATT101200.012.0286561
GTTTTAT144800.011.8149181
GGCGAAT51350.011.53261851
CGAATCG17800.011.0898883
GTTTTTT263650.011.0347051
CGAAAAT34450.010.9143691
GGAAAAT182000.010.8719781
CGCAAAT32950.010.8406691
CGCGTAT5658.0769314E-710.8141591
CGAATAT13850.010.5198561
CGGAAAT44750.010.5027931
GTTTAAT80150.010.3206481
AATCGAA32950.010.2701075
CTTTAAT105400.010.2561671
Produced by FastQC (version 0.11.5)