FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22568227
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG156350.047.49600294
CAAGTCG116250.028.13935594
TAAGTCG42050.026.04280594
GAAGTCG139350.020.9788394
AAAGTCG63300.017.0031694
CGCGTAT4951.0173608E-813.292931
CGGAAAT42700.012.437941
GGCGAAT41050.012.2509131
CGCAAAC41450.011.67913151
CGCGAAC16600.011.3253011
CGCAAAT34150.011.1478771
CGAAGAT91850.011.1039741
GTTTTAT139750.011.0311271
AATCGTA22750.010.94945055
GGAAAAT179400.010.9247481
CTCGGTT39950.010.8235291
CTTTATT163900.010.7821841
GTCGGTT44700.010.7248331
CGAATCG15400.010.6818183
CGCGGAT26900.010.6579931
Produced by FastQC (version 0.11.5)