FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22128725
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126050.056.6013594
CAAGTCG96800.029.52066494
TAAGTCG36200.026.09668494
GAAGTCG140300.023.38275194
TTTTTTC358900.018.5826132
AAAGTCG48350.018.1778794
TTTTTTG388900.016.1218812
GTAACGT27250.015.1779827
CGAAAAT35350.014.7581341
AACGTCG30450.014.6633839
AGTAACG29150.014.5111486
GTTTTTT252200.014.4242661
TTTTTTA317300.013.8496692
TAACGTC34400.012.9796518
GTTTTAC58400.012.9571911
CGCGGAT23950.012.9519831
TACGCTA10800.012.6203699
CGAGTAA33700.012.5519284
GGAAAAC151550.012.4981851
CGCAAAT29300.012.3515361
Produced by FastQC (version 0.11.5)