FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22477804
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG97550.051.5048794
CAAGTCG72350.027.21907294
TAAGTCG30050.023.93011794
GAAGTCG121700.018.65324694
GTCCGAT17050.014.33431151
CGCGTAA10000.014.11
CTCGGTT36950.012.847091
GTTCGAT28900.012.1972311
AAAGTCG48500.011.24123894
TCGGATT27750.011.0090082
GTCGGTT48000.010.6729171
CGCTTAA38950.010.61874244-45
CGCAAAT27950.010.5939171
CTCGGAT64200.010.4688471
AATCGTA24550.010.3380855
TTCGATT33400.010.1317372
GTTTATT110650.010.0244021
CTCAGAT174850.09.9187871
TCGGGTC49300.09.8194722
GTCGGAA35900.09.8189411
Produced by FastQC (version 0.11.5)