FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22477804
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120300.041.6475594
CAAGTCG89950.025.080694
TAAGTCG30500.022.80655794
GAAGTCG125550.017.44484194
CGTATCG16850.013.9465883
TCGGATT27250.013.6256882
GTCCGAT15700.013.1719751
CTCGGTT37800.013.0555561
GTTCGAT29200.011.5890421
CTCGGAT60550.011.4104051
GTCGTTT23150.011.369331
CGCTTAA42000.011.19047544-45
CGCATTT37350.010.8219551
GGCGAAT45500.010.5362631
CGATTGG18800.010.5000014
TCGGTTA14950.010.3745822
GTTTATT111550.010.3227251
CCCGTAT28000.010.2392851
TATACGC9257.2759576E-1110.1621637
CGGTTAT12150.010.0576133
Produced by FastQC (version 0.11.5)