FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23060663
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99500.051.2512694
CAAGTCG78350.025.25462794
TAAGTCG29950.022.59766494
GAAGTCG121900.019.6636694
GTTCGAT33000.013.6727281
GTCCGAT15300.013.2091511
GTCGTTT20900.013.0430631
CGTATCG17950.012.5682463
GTCGGTT55550.011.9297931
TTCGATT34150.011.8360192
AATCGTA21950.011.3485195
AAAGTCG43600.010.99541494
GTCGAAA33800.010.8461541
GTCGGAA38150.010.7182191
GTCGAAT14650.010.5870311
TCGGATT28350.010.1128752
CGCGTAT4651.4776073E-410.1075271
CTCGGAT66900.010.0463391
GGGAAAT166450.09.9675591
GTCGATT22300.09.905831
Produced by FastQC (version 0.11.5)