FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22191660
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG93550.046.47247794
CAAGTCG71450.025.98320694
TAAGTCG27550.020.6424794
GAAGTCG114700.016.39058594
CGAAAAT30450.014.9720861
AAAGTCG47050.014.88416894
TATACGC9150.014.3825157
CGAATCG17350.012.4610953
GTCGGTT50400.012.4027791
TACGCTA9100.011.8791219
CGGAAAT46000.011.6478271
ATATACG13400.011.5746276
CGAAGAT84900.011.4040051
GTTTAAT85500.011.1590651
GTTTATT92950.011.124261
GGAAAAT160550.011.0071631
CTTTAAT89800.010.9910921
GTTTTCT193250.010.9686941
GTTTTTT264700.010.9554211
AATCGTA22600.010.8141595
Produced by FastQC (version 0.11.5)