FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21554309
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT10850.015.1612891
AAATCGA30150.014.9651744
GCGAAAT27700.014.9314091
CTCGATT33000.014.2424251
CGAAAAT40250.014.1291931
GTTTTTT245500.013.5352341
AAAAATC173250.013.1572882
GGAAAAT155000.013.0993561
GTTTAAT79400.012.9042821
CGAAAAC33200.012.8825311
AATCCAC107800.012.8617815
AAATCCA184700.012.7996764
GCCGAAT52150.012.7075751
CGGAAAT42400.012.6367921
TTTAATC91800.012.1339862
CGCAAAT28500.012.0385961
AAAATCC163150.011.9552563
CGGATAT21000.011.6380951
CGCAAAC42950.011.5995341
GAAAAAT185500.011.3762811
Produced by FastQC (version 0.11.5)