FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22815445
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG111100.050.8919994
CAAGTCG84100.024.53388894
TAAGTCG37750.022.65960194
GAAGTCG123050.018.75416694
CGAATCG17300.018.7456653
TACGCTA9750.013.9794869
GGCGAAT39250.013.4114651
AATCGTA24250.013.1793815
TATACGC10000.013.167
GTCGGTT42250.013.0153851
AAAGTCG56450.012.73870794
GCGGTAT28250.012.3115041
CGAAAAT33650.012.2912321
GTTCGAA46100.011.7245121
TCGTAAT23300.011.6995717
GTTTTTT316850.011.5256431
CGAAGAT82050.011.5137111
CGCAAAT31200.011.4487181
CGGAAAT44500.011.4067411
CGCAATT20950.010.9928411
Produced by FastQC (version 0.11.5)