FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22046440
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG169800.044.9240394
CAAGTCG125800.028.5063694
TAAGTCG35700.024.88235394
GAAGTCG146700.021.14519594
AAAGTCG59850.017.51211494
GCGAAAT30050.016.2662221
TATACGC10200.014.7450987
GTTTTTT272300.014.343371
CTCGATT40650.014.3370231
CGCAAAC46700.014.291221
TACGCTA9950.013.6984929
AAAAATC177900.013.6323792
GCCGAAT57000.013.5228071
TTAGCGA22800.013.3991237
GTTTAAT74100.013.3832661
AATCCAC102500.013.3434155
GAAAAAT187650.013.299761
CGAAAAT45100.013.0266081
GGAAAAC123650.012.7715321
AATCGTA14050.012.7117435
Produced by FastQC (version 0.11.5)