FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22230185
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG151600.049.01516794
CAAGTCG115400.026.9618794
TAAGTCG33600.023.91964194
GAAGTCG131500.021.48060894
AAAGTCG55400.017.6462194
GCGAAAT32600.016.4355831
GTTTTTT321900.016.0170861
CGAAAAC34150.015.9648591
CGTGATA23650.014.5073999
GCGGAAT51550.014.2230831
CGCGAAT11600.013.3706881
GTTTAAT93800.013.2281451
GAAAAAT206700.013.1654571
AATCCAC116950.012.5386915
GCCGAAT53350.012.3336461
AAAAATC195500.012.3329912
AAATCCA197900.012.2784234
CGAATCG14600.012.2328763
CTCGATT41550.012.2166061
GGAAAAC134500.012.0208171
Produced by FastQC (version 0.11.5)