FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22101861
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG152050.046.0572294
CAAGTCG114350.026.55181594
TAAGTCG33700.022.45400894
GAAGTCG133150.020.15546494
AAAGTCG62650.015.6041594
GCGAAAT29050.014.5611021
CGCGAAC13350.014.0823981
GTTTTTT278650.013.9827751
GCCGAAT49700.013.4285721
CTCGATT36250.013.0951731
AAATCGA31800.013.006294
GAAAAAT188300.012.9044081
AAAAATC187800.012.8887132
CGAAAAC36950.012.8470911
GTTTAAT84100.012.797861
CGGATAT23750.012.6652641
TCGAATC17450.012.6590272
GCGGAAT41750.012.3832341
CGCAATT22550.012.2971181
AATCCAC115100.012.2502175
Produced by FastQC (version 0.11.5)