FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23031044
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG100700.052.46077794
CAAGTCG73800.026.93902494
TAAGTCG30700.022.045694
GAAGTCG127000.019.35511894
GTTTTTT298600.017.7705961
TTTTTTC384550.015.9253682
CGAAAAT39650.015.7654491
GTTTAAT99600.015.0532121
AAAGTCG46050.014.59500594
TTTTTTG431600.014.5813262
AAATCCA218150.014.2411184
CTCGATT46650.014.1050391
AATCCAC129900.013.6766745
TATACGC7950.013.5974857
CGCAAAT30300.013.4950491
GTTTTAC69600.013.4382181
CTTTAAC79150.013.3013271
GAAAAAT241650.013.0701431
AAAAATC233750.013.0695192
GTTTTTC162300.013.0024631
Produced by FastQC (version 0.11.5)