FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23031044
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127250.041.69980294
CAAGTCG93250.023.13458494
TAAGTCG32350.022.95517794
GAAGTCG124300.016.97747294
GTTTTTT324550.015.3504851
GCGAAAT26650.014.2851781
CTTAGAT60650.014.25886251
CTCGATT47750.013.7801051
AAATCGA31300.013.6645364
AAATCCA204900.013.5793074
CGAAAAC34400.013.1162791
AAAGTCG56900.012.88576494
CTCAGAT170500.012.625221
AATCCAC126050.012.4537885
CGCAAAT32100.012.4454831
AAAAATC213850.012.4395612
TATACGC9100.012.3956047
CGAATCG16700.012.3832343
GGAAAAT166750.012.2326831
GTTTAAT91250.011.9495891
Produced by FastQC (version 0.11.5)