FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22147399
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131200.060.6486394
CAAGTCG105700.031.88173994
TAAGTCG37550.026.41011894
GAAGTCG143000.023.2699394
AAAGTCG53000.017.02641394
GTTTTTT290300.016.335861
TTTTTTC375600.014.4403612
GCGAAAT27300.013.9450531
GCGGAAT42250.013.9053251
GCCGAAT51100.013.7964761
AAATCGA32450.013.6147924
GAAAAAT211250.013.5938451
GTTTAAT90850.013.554211
TCGAATC20450.013.3300722
TACGCTA8850.013.2768359
AAAAATC206500.012.9961252
GTTTTAT158250.012.4739341
TTTTTTG411350.012.4426872
GTTTTTC161350.012.2342731
CGCAAAT34300.012.1953351
Produced by FastQC (version 0.11.5)