FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22147399
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG159050.049.70386594
CAAGTCG122250.028.02699394
TAAGTCG38800.022.28865894
GAAGTCG141900.021.5954994
AAAGTCG62350.017.78989494
GCCGAAT49150.017.2126141
GTTTTTT276850.015.1941471
CGCATAT7350.014.7074821
GCGAAAT25900.013.6100381
CGAAAAC36000.013.0555551
AAATCCA186050.012.3783924
TATACGC9500.012.3684217
CTCGATT31600.012.1962011
GTTTAAT75950.012.1290321
AATCGAG34700.012.0547545
GCGAATT21400.011.8598131
GAAAAAT201150.011.7295541
GTTTTAT141250.011.6460171
AAATCGA31900.011.6394974
AATCCAC110050.011.5738295
Produced by FastQC (version 0.11.5)