FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22325584
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG48650.020.57759594
CGAAAAT40000.016.5675011
GTTTTTT342550.015.9845281
GCGAAAT28250.015.805311
CTCGATT50200.015.7290821
TTTTTTC448500.015.09030152
TTTTTTG498600.013.9039312
CGCGAAT15300.013.8235281
GTTTTAC82650.013.5910451
GTTTAAT114900.013.5395991
CGCAAAT30550.013.5384611
AAAAATC271700.013.1641512
GAAAAAT284100.013.0362551
AAATCCA221050.012.7572944
CGTATCG18950.012.649075550-51
TTTTTTA485100.012.4596992
CTTTAAC87800.012.4191341
TCGAATC19800.012.3434332
TCGATTC50550.012.0870422
GTTTTAT200050.012.0054981
Produced by FastQC (version 0.11.5)