FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22325584
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA7000.018.128579
CTCGATT51800.015.4247091
CGCAAAC42050.014.9774071
TATACGC8000.014.68757
CGAAAAC34600.014.670521
GTCGAAT12250.014.5795911
CGAAAAT50150.014.43271
GTTTTTT349100.014.4056141
AAGTCGG69750.014.28530494
CGGATAT23950.013.3444661
CTCAGAT170950.013.1418521
GTTTTAC87450.012.7913081
GTTTAAT106950.012.78821851
AAAAATC250900.012.7568752
GCGAAAT26900.012.7546461
AAATCCA223350.012.7521824
CTTAGAT82700.012.55985451
TCGATTC54850.012.167732
CTTTAAC91000.011.6208791
TCGAATC19900.011.5728652
Produced by FastQC (version 0.11.5)