FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22622346
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116400.054.67181894
CAAGTCG90700.027.87872194
TAAGTCG30150.022.13598694
GAAGTCG134100.020.53840494
AAAGTCG48150.016.10591994
GTTTTTT294050.015.392281
TTTTTTC367200.013.8491282
GCGAAAT28550.013.33451
GTTTTTC151750.012.9462931
GTTTTAC61850.012.766371
GCCGAAT50800.012.6751971
CCGAATC59150.012.0777682
GCGGAAT38400.011.9947921
CGAATCC41200.011.9781553
AAATCCA193050.011.9538974
GGAAAAT162000.011.9530861
AAATCGA31900.011.9341694
GAAAAAT213000.011.8934271
TTTTTTG411550.011.842792
GTTTTCT210200.011.82825851
Produced by FastQC (version 0.11.5)