FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22622346
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137150.045.98906794
CAAGTCG106550.026.95166894
TAAGTCG31500.024.17142994
GAAGTCG123250.018.38052794
GCGAAAT26950.015.6957321
GCCGAAT46700.015.3982871
GTTTTTT270900.013.6888151
CGAAAAC36800.013.5380431
AAATCGA30100.013.2724254
CTCGATT36000.013.1861111
AAAGTCG59500.013.03361394
CGGAAAT43100.012.4315551
AAAAATC196200.012.3608572
GAAAAAT196750.011.9679791
CGCGAAC17050.011.5777131
CGCAAAC45500.011.5692311
AAATCCA192250.011.5146944
AATCCAC110350.011.1164475
GTTTTAC63750.011.0588241
GCGATTT46900.011.0234541
Produced by FastQC (version 0.11.5)