FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22619056
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG151600.044.8608294
CAAGTCG112150.028.246194
TAAGTCG40100.023.55860794
GAAGTCG139350.019.29242994
CGCGTAT5650.016.6371691
AAAGTCG68450.014.76260294
CGAATCG14400.014.0347223
CGAGAAC69250.013.4382681
CGCAAAC48700.012.7392211
AATCGTA17100.012.6432765
CGGACAT59400.012.2643111
CGAAGAT84600.012.0555551
CTTTAAT109800.011.8570131
CGGAAAT58000.011.7500011
GTTTTTT301100.011.7070741
CGAAAAT48800.011.6536881
CGCTAAT9800.011.5102041
CTCGTTT42550.011.3772041
GTCCGAT16200.011.31481551
TCGGATT36450.011.2181072
Produced by FastQC (version 0.11.5)