FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22026532
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG144250.063.01421494
CAAGTCG115800.032.5103694
TAAGTCG37800.029.5925994
GAAGTCG160000.025.93812694
AAAGTCG54250.019.40645294
GTTTTTT293750.016.0479981
TTTTTTC359800.014.7217892
CGAAAAT35450.013.5232721
GAAAAAT204150.013.2147931
AAAAATC192950.012.9831562
CGCAAAT33100.012.7794561
TTTTTTG395650.012.2949572
GCGAAAT26950.012.0333951
GTTTAAT93050.011.97098351
CGTATCG16450.011.85714250-51
AATCCAC112700.011.6770185
GTTTTAC59300.011.4924121
GTTTTAT152100.011.3096651
CGGAAAT46600.011.2961381
CGTAGCG41800.011.01913830-31
Produced by FastQC (version 0.11.5)