FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22026532
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG170650.053.2382194
TAAGTCG38700.029.26873694
CAAGTCG133950.029.0526394
GAAGTCG154200.025.60311394
TACGCTA9050.017.657469
AAAGTCG63400.017.12460794
TATACGC8700.015.6666677
GTTTTTT278900.014.5600571
ATATACG12200.013.8688536
CGCGAAT7700.013.4285711
CGAAAAT43050.013.3193961
CGCATAT8300.013.02409651
GCCGATT21500.012.0232551
CGAAGAT79600.011.9271351
CTTTAAT96600.011.7256731
GTTTAAT83850.011.6028621
CGCAAAC44350.011.3393461
CGCGTTT14100.011.3333331
AAAAATC184350.011.0138322
GTTTTCT204500.010.9628371
Produced by FastQC (version 0.11.5)