FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22738370
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG151150.045.67846794
CAAGTCG113600.026.97535194
TAAGTCG38600.026.54404394
GAAGTCG131350.019.68024394
AAAGTCG64350.015.33799594
CGAAAAT42200.012.0284361
TTAGCGA18400.012.0054357
CGCGAAT17300.011.9537561
CGCAAAC46050.011.8393041
GGAAAAT178850.011.6941571
TACGCTA9350.011.5614979
CGCAAAT35000.011.5485711
TTTAGCG21250.011.5011762
GTCGGTT44700.011.3557031
CTCGTTT41450.011.2255721
TTAGCGC18450.011.2086723
AATCGTA18950.011.1609495
CGGAAAT46500.011.11827851
AATCGGG41100.011.0924575
GGCGATT36600.010.7868851
Produced by FastQC (version 0.11.5)